ELISA ASSAY Essay Example | Topics and Well Written Essays - 500 words

ELISA ASSAY - Essay ExampleIn this graph various dilution of walk anti hare Ig G antibodies were used to determined tightness of lapin Ig G. the close effective dilution volition be between 1/4000 to 1/8000, as in rest of the boldnesss, at lower concentration of Ig G there is no linearity with increasing concentration of rabbit immunoglobulin G (like in case of 1/2000). At1/2000 dilution concentration of mouse anti-rabbit Ig G is too gritty compared to rabbit IgG and hence there is no linier relation. Similarly at more than 1/8000 dilution concentration of anti rabbit IgG becomes to less compared to concentration of rabbit Ig G. hence there will not be any anti rabbit Ig G available to bind with rabbit Ig G at high concentration. In given scenario the optimum dilution for mouse anti- rabbit IgG will be 1/6000 which gives linear correlation with increase in concentration of rabbit IgG. here(predicate) in case of goat anti rabbit Ig G the over all bind and absorption profiles r estricts it use for estimation of rabbit IgG. There are two different scenario 1) In case of lower dilution (1/2000) there is lots of non specific binding resulted in to very high absorption at lower concentration of rabbit Ig G and get saturated at slightly higher concentrations of rabbit Ig G.2) In case of higher dilution (1/4000 and above) there is very less binding repayable to non specificity of polyclonal anti body.